d351235422 Baker; J. The resin must then be carefully washed 0.5% DIPEA in DMF, 3 10 ml of 0.5% sodium diethylthiocarbamate in DMF, and then 5 10 ml of 1:1 DCM:DMF. A case of debenzylation by catalytic hydrogenolysis at sulfonylated nitrogens after Boc-substitution has also been reported.28 Thus, TsNHBoc was obtained from TsNBn(Boc) in essentially quantitative yield, obviously also due to strong Boc/benzyl interaction in the substrate. Another method is fragment condensation, in which peptide fragments are coupled. Bull. Due to the complex nature of in vitro protein synthesis, the addition of amino acids to the growing peptide chain occurs in a precise, step-wise and cyclic manner.
Newer developments omit the carbodiimides totally. This was demonstrated by selective cleavage of N-acetyl from the primary amino groups of spermidine.15. 2,3-Diaminoproanoic acid and 2,4-diaminobutanoic acid derivatives are commercially available. These problems can be greatly reduced by protecting the indole nitrogen. Murugan, Arkivoc, 2001, 201226CAS.A Reaxys search (June 2013) for R-CO-N-R(Boc), both R and R any group except hydrogen, corrected for R-N(Boc)2 below that are obviously included, gave 7800 5600 = 2200 hits.L. doi:10.1146/annurev.biophys.34.040204.144700. Contents 1 Chemistry 2 Liquid-phase synthesis 3 Solid-phase synthesis 3.1 BOP SPPS 4 Solid supports 4.1 Polystyrene resin 4.2 Polyamide resin 4.3 PEG hybrid polystyrene resin 4.4 PEG-based resin 5 Protecting groups 5.1 N-terminal protecting groups 5.1.1 t-Boc protecting group 5.1.2 Fmoc protecting group 5.1.3 Comparison of Boc and Fmoc solid-phase peptide synthesis 5.1.4 Benzyloxy-carbonyl (Z) group 5.1.5 Alloc protecting group 5.1.6 Lithographic protecting groups 5.2 Side chain protecting groups 5.3 Protection schemes 6 Activating groups 6.1 Carbodiimides 6.2 Triazoles 7 Regioselective disulfide formation 8 Synthesizing long peptides 9 Coupling efficiency vs.
Unyttakiy replied
478 weeks ago